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HeLa cells grown on sterile cover slips were treated with IC50 doses of AMEs for 24 hours. Then they were stained with Acridine orange (1 μg/ml) to study the formation of acidic vacuoles. Scale indicates 50 μm. A-D) Non-treated, E-H) Vehicle control, I-L) E. intestinalis extract treated, M-P) R. riparium treated HeLa cells.
