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. 2014 Jan 15;12:4. doi: 10.1186/1477-5956-12-4

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Copyright © 2014 Wang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Linearity and accuracy of the self-build quantitative method in glycopeptides from glycoprotein standards. Four glycopeptides, FATN*TTLTK and LMTN*ETSDRPLVHFTPNK from invertase [Swiss-Prot:P00724] and AESN*GSYLQLVEISR and LAPLN*DSR from Fetuin [Swiss-Prot:P12763], were serially diluted to determine the linearity and accuracy of the self-build quantitative method. On the left are the results from manual calculation and on the right are the results from the self-build quantitative method. Glycopeptide concentration ratios calculated manually and those from the self-build method were all significantly associated with the theoretical values, with both correlation coefficients (R²) being >0.99. *denotes the N-glycosylation site.