Figure 3. MT recognition of Ska1 via its multipartite mode of MT binding is a functional requirement for the Ska complex.

(a) Box-and-whisker plot showing the elapsed time (min) between nuclear envelope breakdown (NEBD) and anaphase onset/death for individual cells. The total number of cells (n) from two or more independent experiments is given above each box. Lower and upper whiskers represent 10th and 90th percentiles, respectively. Table summarizing information from the live cell experiments shown below regarding the average time in mitosis (from NEBD until anaphase onset/cell death) and the percentage of cells dying in mitosis. (b) Representative stills from time-lapse video-microscopy experiments illustrating mitotic progression of HeLa S3 cells stably expressing histone H2B-GFP. Scale bar, 10 μm.