Fig. 1.

MECs cell proliferation is induced by prostate tumor conditioned media (TCM). TCM were collected from LAPC-4 and LNCaP prostate tumor cell cultures after incubating for 24, 48, and 72 hr, respectively, as described in the Materials and Methods Section. In (A), MECs plated in 96-well plates were treated with control media (0), or 50% TCM collected from 48-hr LAPC-4 cell cultures for 24 or 28 hr. In (B,C), MECs plated in 96-well plates were treated for 48 hr with control media (0), or 10%, or 50% of TCM collected from 24, 48, and 72-hr LAPC-4 (B) or LNCaP (C) cell cultures. The viable cells number was determined at the end of experiments, and the data are expressed as fold of corresponding media control of each experiment. Results are means ± SEM of three independent triplicate experiments. **P < 0.01 between the indicated groups; ^##P < 0.01 compared to corresponding 10% TCM-24 hr and 10% TCM-48 hr group; and ^@@P < 0.01 compared to corresponding 50% TCM-24 hr and 50% TCM-48 hrgroup.