B6 mice were immunized with 104
Py either as RAS or ITV with 10 days CQ chemoprophylaxis. Arrows indicate day of vaccination or rechallenge. (A), Representative CD8 T cell responses in blood at the indicated days post vaccination. Numbers indicate the frequency of CD8 T cells exhibiting an antigen-experienced (CD8αint, CD11ahi) phenotype. (B), Cumulative and kinetic analysis of ITV versus RAS vaccine-induced CD8 T cell responses. Secondary effector responses were measured after administration of 104 virulent sporozoites on day 68. Data are Mean +/− SD for 30 mice (through d68) and 10 mice (d68 to d88). **, P <0.0001; *, P = 0.00143.