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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Biomaterials. 2014 Jan 15;35(10):3243–3251. doi: 10.1016/j.biomaterials.2013.12.081

Fig. 4.

Fig. 4

Fluorescence micrographs of cells grown inside or outside fibrin hydrogel microfibres. (A–B) ASCs with CellTracker™ green CMFDA dye were encapsulated in 0.75 wt% fibrin hydrogel fibres. After 2 days (a) and 5 days (b) of culture, cells spread out showing alignment along the fibre axis. (C–D) F-actin filament (green) staining of human ECFCs cultured on the surface of a rehydrated fibrin microfibre, overlapped with immunofluorescence staining for CD31 (red) (C) or vWF (red) (D) and DAPI (blue) staining for cell nuclei. (E) A side view and a cross-section view of the sample shown in (C–D), indicating that the differentiated ECFCs grew into a confluent tubular structure.