Table 1.
Summary of mutational analysisa
| EnvC variant |
LytEnvC lytic activity |
LytEnvC protein stability |
FLEnvC function |
FLEnvC septal localization |
LytEnvC amidase activationd (%) |
|---|---|---|---|---|---|
| wild type | + | + | + | + | 100.00 |
| H308Ac | + | ND | ND | ND | ND |
| W320Ac | + | ND | ND | ND | ND |
| K321Ac | + | ND | ND | ND | ND |
| V324Ac | + | ND | ND | ND | ND |
| R381Ec | + | ND | ND | ND | ND |
| Y401Fc | + | ND | ND | ND | ND |
| K321Ec | − | + | − | + | 7.12 |
| V324Ec | − | + | − | + | ND |
| Y350Ac | − | + | − | + | 22.31 |
| V353Ab | − | + | − | + | 10.04 |
| R405Hb | − | + | − | + | 4.73 |
| Y366Hc | − | +/− | ND | ND | ND |
| Y401Ec | − | +/− | − | + | ND |
| W320Ec | − | − | ND | ND | ND |
| D338Kc | − | − | ND | ND | ND |
| V355Ec | − | − | ND | ND | ND |
| L400Pb | − | − | ND | ND | ND |
| A410Ec | − | − | ND | ND | ND |
| N412Kb | − | − | ND | ND | ND |
a
Rows highlighted in green, pink, blue, or grey denote variants that are functional, non-functional/stable, non-functional/partially stable, or unstable, respctively.
b
Indicates a variant obtained by random selection.
c
Indicates a variant obtained by site directed mutagenesis.
d
Values represent the percentage of AmiB activity observed in reactions containingLytEnvC variants relative to wild-type after 30 minutes (see Figure 6)