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. Author manuscript; available in PMC: 2014 Feb 13.
Published in final edited form as: Eur J Immunol. 2012 Dec 27;43(2):521–532. doi: 10.1002/eji.201242757

Figure 2. JE131 cells have a DN3 pre-T cell phenotype.

Figure 2

(A) Non-transduced JE131 cells or B6 splenocytes were stained for CD44 or CD25 and analyzed by flow cytometry (left). Non-transduced JE131 cells were co-stained for TCR-Cβ and pre-Tα and analyzed by flow cytometry (right, top). Pre-Tα expression level of each population (gated on live and pre-TCR+ or αβTCR+ cells) is also shown (right, bottom). Data are representative of two independent experiments. (B) PCRs to amplify Ptcra were performed on cDNA prepared from JE131 cells. As positive or negative controls, PCRs were performed on cDNA prepared from TCRα−/− mouse thymocytes or the plasmacytoma cell line uM2. Data are the results of one experiment. (C) JE131 cells were transduced with empty MiT vector or MiT-Ikaros retrovirus. 3 days post-transduction, cells were co-stained for TCR-Cβ and pre-Tα and analyzed by flow cytometry. Plots (gated on live cells and different levels of Thy1.1 expression) show TCR-Cβ vs. pre-Tα expression. Data are representative of two independent experiments.