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. Author manuscript; available in PMC: 2014 Feb 13.
Published in final edited form as: J Biol Chem. 2005 Feb 17;280(17):16962–16968. doi: 10.1074/jbc.M409693200

Fig. 3. PKA regulates surface AMPA receptor levels.

Fig. 3

A, schematic of the experimental protocol. Cultures were treated with NMDA for 3 min, returned to the incubator for 27 min, and then treated with forskolin (forsk) to activate PKA. B, representative immunoblot for surface GluR1 showing that forskolin reverses the NMDA-induced loss of surface GluR1. C, NMDA reduces surface GluR1 to 48 ± 7% controls. Forskolin significantly reverses this effect (83 ± 8%; n = 8; *, p < 0.05 versus NMDA alone, Student's t test). D, blocking PKA activity with Rp-cAMPS (Rp) causes a reduction in surface GluR1. E, Rp-cAMPs significantly reduces surface GluR1 to 82 ± 6% control levels. (n = 6; *, p < 0.05 versus control, Student's t test).