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. 2014 Jan 8;13(2):1147–1155. doi: 10.1021/pr4009892

Figure 5.

Figure 5

Analysis of the TMT data. (A) Venn diagram of the number of proteins identified in the SH-TBK1_MOUSE protein complex from the three elution conditions: 100 mM formic acid, 100 mM glycine, and 2% SDS. Only proteins identified with at least two unique peptides and that were more than >3-fold higher compared with the corresponding SH-GFP elution (channels 126, 127, and 128 TMT 6-plex) are included. (B) TMT ratios of the SH-TBK1 interactors resulting from the intersection of the three elution conditions assessed. All ratios were obtained by comparison with the formic acid elution of the SH-TBK1 TAP (channel 129 of the TMT 6-plex). SDS, sodium dodecylsulphate.