Table 1.
Relative ATP citrate lyase mRNA levels in human and rat islets are much higher than in liver of the same genus.
| Genus (n) | Acly/Glud1 | Acly/18s |
|---|---|---|
| Young rat livers (2) | 1 (1.01, 0.99) | 1 (1.07, 0.93) |
| Old rat livers (2) | 4.4 (4.5, 4.3) | 5.4 (5.0, 5.7) |
| Average all rat livers (4) | 2.7 ± 0.9 | 3.2 ± 1.1 |
| Rat islets (4) | 30 ± 4.4a | 7.3 ± 1.8 |
| Rat islet/Rat liver (4) | 11 | 2.3 |
| Human livers (2) | 1 (083, 1.17) | 1 (0.53, 1.47) |
| Human islets (9) | 49 ± 7.8a | 30 ± 2.7a |
Islet AcLy mRNA was estimated by qRT-PCR and is expressed relative to values from liver samples from 4 rats (two 7-week old male rats (weighing 200 g and 207 g) and two 11-month-old female retired breeder rats (weighing 327 g and 339 g)) or the average of 2 human liver samples. RNA was isolated from 4 batches of islets with each batch from eight 200–250 g rats. The mean ± SE BMI and age of the 9 human donors whose islets were used for the mRNA measurements were 30 ± 1.8 kg/m 2 and 46.3 ± 5.4 years. Values were normalized to glutamate dehydrogenase (Glud1) mRNA and 18 s RNA as internal controls. The average relative Acly mRNA levels of livers from the younger rats and human livers were assigned a value of 1 and the levels of other livers and islets are expressed relative to these averages. Results are the mean ± SE with the number of samples in parentheses for n > 2. Individual values are shown in parentheses when there were 2 samples.
p < 0.001 vs. same genus average liver value.