Figure 2.

Comparison of oligo(dT)₂₅ immobilization efficiency and oligo(dA)₂₅ capture efficiency by oligo(dT)₂₅ on carboxylated channel surfaces and dendrimer-modified channel surfaces. Fluroescently-labelled oligo(dT)₂₅ was immobilized on carboxylated surfaces containing no dendrimers and dendrimer-modified surfaces, and the fluorescence intensities were compared for the different surfaces (A). Fluorescently-labelled oligo(dA)₂₅ (complimentary target) was captured on both carboxylated and dendrimer-modified surfaces with immobilized oligo(dT)₂₅ capture probes, and the fluorescence was quantified (B: blue bars). Fluorescent oligo(dT)₂₅ (non-complimentary target) was also pumped through both of these channels to determine the non-specific binding to each type of surface (B: red bars).