Fig. 1.

Targeted disruption of the mouse miR29ab1 gene. The structures of the wild-type allele and the disrupted allele are shown. The targeting vector was constructed from two homologous recombination arms, a 5′ one of 4171 bps and a 3′ one of 3857 bps, and a 600 bp genomic fragment containing miR29a and miR29b1 cloned in between two loxP sites. Genomic DNA was used for Southern blot analysis after digestion with SacI and labelled with a 3′ probe. Recombinant clones exhibited two bands: a wild-type one of 5.8 kb and a mutant one of 7.4 kb.