Fig. 3.

Hypoxia inhibited early activation of T cells in vivo even in the absence of A2AR. WT and A2AR^−/− mice received injection of anti-CD3 mAb while breathing a normoxic or hypoxic (8% oxygen) atmosphere. Changes in CD69 (A) and CD25 (B) expression on T cells in the spleen were analyzed 2h after the injection of anti-CD3 mAb. Percentages of CD69-positive or CD25-positive cells in the CD4⁺ or CD8⁺ population are shown. Data represent average ± SD of 3–5 mice. a, P < 0.05; b, P < 0.01; c, P < 0.001 versus anti-CD3 mAb + normoxia.