Table 2.
Effect of over-expressing genes involved in NADPH synthesis on SA production in E. coli SA112
| Strain | OD 600 | SA concentration (g/L) | Yield (mol SA/mol glucose) | NADPH (μM) |
|---|---|---|---|---|
| SA112 (pMP5) |
4.79 ± 0.64 |
1.34 ± 0.09 |
0.22 |
1.93 ± 0.01 |
| SA112 (pMPsthA) |
5.78 ± 0.26 |
1.42 ± 0.07 |
0.24 |
1.61 ± 0.01 |
| SA112 (pMPpntAB) |
5.50 ± 0.11 |
1.89 ± 0.01 |
0.27 |
2.23 ± 0.01 |
| SA112 (pMPnadK) |
5.20 ± 0.34 |
1.92 ± 0.01 |
0.29 |
2.55 ± 0.01 |
| SA114 |
6.55 ± 0.30 |
2.99 ± 0.01 |
0.31 |
4.51 ± 0.01 |
| SA116 | 6.40 ± 0.51 | 3.12 ± 0.01 | 0.33 | 5.77 ± 0.01 |