Table 5.
Primers used in this study
| Primer | Sequence and purpose a |
|---|---|
| LP1 |
5’-ATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACAGCGATTGTGTAGGCTGGAG-3’, deletion of aroL |
| LP2 |
5’-TCAACAATTGATCGTCTGTGCCAGGGCGCTGCGAATTTCAGAAATCACCTTAACGGCTGACATGGGAATTAG-3’, deletion of aroL |
| P1 |
5’-GTTTCGTTGGCATCGTTCTT-3’, Diagnostic PCR for the deletion of aroL |
| P2 |
5’-ATTCTCATGACACCGGCTTT-3’, Diagnostic PCR for the deletion of aroL |
| KP1 |
5’-ATGGCAGAGAAACGCAATATCTTTCTGGTTGGGCCTATGGGTGCCGGAAAAGCGATTGTGTAGGCTGGAG-3’, deletion of aroK |
| KP2 |
5’-TTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTAACGGCTGACATGGGAATTAG-3’, deletion of aroL |
| P3 |
5’-GCGAAGCGGGTTTATCATTA-3’, Diagnostic PCR for the deletion of aroK |
| P4 |
5’-GTTCCCCGAGAGTAACGAC-3’, Diagnostic PCR for the deletion of aroK |
| GP1 |
5’-GCCTGCAGAGGAGGGCGTAAATATGAATTATCAGAACGACGATT-3’, PstI, PCR for aroG |
| GP2 |
5’-CGGCATGCTTACCCGCGACGCGCTTTTACT-3’, SphI, PCR for aroG |
| G15P1 |
5’-CTCAATATGATCACCCCACAAT-3’, sited-specific mutagenesis of aroG |
| G15P2 |
5’-AAACTCACCTGCCGCTGGCAGACCG-3’, sited-specific mutagenesis of aroG |
| EP1 |
5’-GCGAATTCAGGAGGTAATAAATATGGAAACCTAT GCTGTTTT-3’, EcoRI, PCR for aroE |
| EP2 |
5’-CGGCGGCCGCTTATCACGCGGACAATTCCTCCT-3’, NotI, PCR for aroE |
| BP1 |
5’-CGGCGGCCGCAGGAGGTAATAAATATGGAGAGGATTGTCGTTAC-3’, NotI, PCR for aroB |
| BP2 |
5’-GCCCATGGTTACGCTGATTGACAATCGG-3’, NcoI, PCR for aroB |
| TP1 |
5’-GCGCATGCAGGAGGTAATAAATATGTCCTCACG TAAAGAGCT-3’, SphI, PCR for tktA |
| TP2 |
5’-CGGAGCTCTTACAGCAGTTCTTTTGCTTTCG-3’, SacI, PCR for tktA |
| PntF |
5’-CAGGGTACCTCATCAATAAAACCG-3’, KpnI, PCR for pntAB |
| PntR |
5’-CGTCTGCAGTTACAGAGCTTTCAG-3’, PstI, PCR for pntAB |
| SthF |
5’-TTTTGGTACCCAGGTAAGCCCTACCATGC-3’, KpnI, PCR for sthA |
| SthR |
5’-GGGCTGCAGGGCCATTTCGATAAAGTTTT-3’, PstI, PCR for sthA |
| NadF |
5’-GCGGGGTACCATGAATAATCATTTCAAGTG-3’, KpnI, PCR for nadK |
| NAdR |
5’-GCGGTCTAGATTAGAATAATTTTTTTGACCA-3’, XbaI, PCR for nadK |
| 5TacF |
5’-ACGCGTGTAAAACGACGGCCAGT-3’, MluI, PCR for the 5Ptac promoter |
| 5TacR |
5'-CCGCGCATGCGGATCCGAATTCATGCATCTAGTATTTCTCCTCTTTAATGGAT-3’, SphI, PCR for the 5Ptac promoter |
| CsrF |
5’-CGGAGCTCAGGAGGTAATAAATGAGTCAGACAACGAAGTGAACAT-3’, SacI, PCR for the csrB and pps gene cluster |
| PpsR |
5’-CGGGTACCTTATTTCTTCAGTTCAGCCAGG-3’, KpnI, PCR for the csrB and pps gene cluster |
| QEF |
5’-GGATCGCCGGAATATCACCAC-3’, qPCR for the aroE gene |
| QER |
5’-ACTACTGCCACTCCTTTCCCT-3’, qPCR for the aroE gene |
| QBF |
5’-AAC GAA ACC CTG GCT CCT CTG-3’, qPCR for the aroB gene |
| QBR |
5’-AAGCGCCACCAGCGTAGTATC-3’, qPCR for the aroB gene |
| QGF |
5’-CGTTGCTGAAGTGAAAGAAGGG-3’, qPCR for the aroG gene |
| QGR |
5’-ACGTCAGCACAAACATCCATC-3’, qPCR for the aroG gene |
| QTF |
5’-TTTCGCCTGGCCTGCTTCTTT-3’, qPCR for the tktA gene |
| QTR |
5’-CGACGCTGAAATTGCCCTGAC-3’, qPCR for the tktA gene |
| QNF |
5’-TGGAATCAACCGTGGCAACCT-3’, qPCR for the nakKgene |
| QNR |
5’-TGGAATCAACCGTGGCAACCT-3’, qPCR for the nakKgene |
| QPF |
5’-AGCCGGAGTACGAGTTCAGCA-3’, qPCR for the pntAB gene |
| QPR |
5’-ATTGCGCTGGTATTCGGCTGG-3’, qPCR for the pntAB gene |
| QPPF |
5’-GACATCTTCTCGCTGACCAAC-3’, qPCR for the ppsA gene |
| QPPR |
5’-TTACCGGTGTGGCCATCTTTC-3’, qPCR for the ppsA gene |
| QCSF |
5’-CTGGATGAAGCGAAGAGGATG-3’ , qPCR for the csrB gene |
| QCSR |
5’-ATTGCTTCCTGCTCACACCAC-3’, qPCR for the csrB gene |
| QCF |
5’-TTGTCGGCGGTGGTGATGTC-3’, qPCR for the cysG gene |
| QCR | 5’-ATGCGGTGAACTGTGGAATAAACG-3’, qPCR for the cysG gene |
aRestriction enzyme sites are underlined.