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GC-MS analysis of sabinene from the headspace of the sealed cultures of strain HB2. Cultures were induced at 37°C, OD600 = 0.6-0.9, and final concentration of 0.25 mM IPTG. By comparing with the authoritative sabinene (A, B), the capacities of sabinene biosynthesis were verified. A, C, total ion chromatogram (TIC); C, D, mass spectrum. Based on the relative retention time and total ion mass spectral comparison with an external standard, sabinene production was identified.
