Table 1.
Plasmids and strains used in this study
| Name | Relevant characteristics | References |
|---|---|---|
|
Plasmids |
|
|
| pACYCDuet-1 |
P15A origin; CmR; PT7 |
Novagen |
| pTrcHis2B |
ColE1 origin; AmpR; Ptrc |
Invitrogen |
| pGH |
pUC origin; AmpR; PT7 |
Generay |
| pTrcLower |
ColE1 origin; AmpR; Ptrc:: ERG12-ERG8-ERG19-IDI1 |
[42] |
| pHB1 |
P15A origin; CmR; PT7:: SabS1 |
This work |
| pHB2 |
P15A origin; CmR; PT7::IspA |
This work |
| pHB3 |
P15A origin; CmR; PT7::IspA-SabS1 |
This work |
| pHB4 |
P15A origin; CmR; PT7:: GPPS2 |
This work |
| pHB5 |
P15A origin; CmR; PT7:: GPPS2-SabS1 |
This work |
| pHB6 |
P15A origin; CmR; PT7:: mvaE-GPPS2-SabS1 |
|
| pHB7 |
P15A origin; CmR; PT7::mvaE-mvaS-GPPS2-SabS1 |
This work |
|
Strains |
|
|
|
E. coli BL21(DE3) |
E. coli B dcm ompT hsdS(rB - mB -) gal |
Takara |
|
E. coli DH5α |
deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 |
Invitrogen |
|
Saccharomyces cerevisiae |
Type strain |
ATCC 204508 |
| HB1 |
E. coli BL21(DE3) harboring pHB1 |
This work |
| HB2 |
E. coli BL21(DE3) harboring pHB3 |
This work |
| HB3 |
E. coli BL21(DE3) harboring pHB5 |
This work |
| HB4 | E. coli BL21(DE3) harboring pHB7 and pTrcLower | This work |