FIGURE 1:
Generation of ERdj4GT/GT mice. (A) The GT allele. The GT cassette was inserted between adenosine 1151 and guanosine 1152 within intron 1 of the ERdj4 locus (NC_000078.6). Quantitative RT (qRT)-PCR was performed using primers specific for β-galactosidase (a, b) and ERdj4 (c, d). β-GEO, β-galactosidase/neomycin resistance fusion gene; IRES, internal ribosome entry site; pA, polyadenylation signal; PLAP, placental alkaline phosphatase; SA, splicing acceptor; TM, transmembrane region. (B) PCR genotyping of the WT and GT alleles in gDNA isolated from the progeny of heterozygous intercrosses. (C) GT copy number was determined in gDNA by the TaqMan Gene Copy Number assay. n = 4 mice/genotype. (D, E) qRT-PCR of β-galactosidase and ERdj4 mRNAs in MEFs. RQ, relative quantitation. n = 3 samples/group. (F) qRT-PCR of ERdj4 mRNA in tissues isolated from 6-wk-old littermates; samples were normalized to β-actin. n = 4 mice/genotype.