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. 2014 Feb 15;25(4):495–507. doi: 10.1091/mbc.E13-09-0526

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© 2014 Klinger et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — SSX2IP is a centriolar satellite protein localizing to basal bodies in ciliated cells. (a) Indirect immunofluorescence of serum-starved RPE-1 cells with specific antibodies as indicated; glu-tub, glutamylated tubulin. (b) Indirect immunofluorescence in NIH3T3 cells stably expressing LAP-SSX2IP (note that the LAP tag contains GFP), which was visualized with GFP antibodies. (c, d) Immunoprecipitation with single-chain GFP antibodies of lysates from serum-starved NIH3T3 cells and serum-starved NIH3T3 cells stably expressing LAP-SSX2IP. (c) Quantitative mass spectrometry analysis of immunoprecipitates. The N-termini of tryptic peptides were dimethylated using stable carbon isotopes for relative quantification of peptide abundance in SSX2IP immunoprecipitates (light) vs. control samples (medium). (d) Immunoblot shows coimmunoprecipitation of SSX2IP and PCM-1.