FIGURE 4:

Cep290 recruitment to centrosomal satellites and basal bodies depends on SSX2IP in RPE-1 cells. (a) Cells were transfected with control or SSX2IP siRNA and stained for Cep290 and γ-tubulin using indirect immunofluorescence. (b) Average projection of 50 cells immunostained as shown in a. Centering of single images before projection was based on the γ-tubulin signal. (c, d) Quantification of signal intensities of Cep290 at the basal body (c) or in satellites (d) of cells transfected with control or SSX2IP siRNA. (c, d) Left (bars), mean values of averages ± SEM from three independent experiments (n = 150) normalized to controls. Right (box-and-whiskers plots), quantification of a single representative experiment. *p < 0.05, **p < 0.01, ***p < 0.001 (two-tailed Student's t test).