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. 2014 Feb 15;25(4):495–507. doi: 10.1091/mbc.E13-09-0526

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© 2014 Klinger et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — SSX2IP is required for ciliary BBSome localization in RPE-1 cells. (a) Cells were transfected with control or SSX2IPs siRNA and stained for BBS4 or γ-tubulin and glutamylated (glu) tubulin using indirect immunofluorescence. (b) Quantification of immunofluorescence shown in a. Cells with cilia showing ciliary localization of BBS4 were determined in relation to the overall cell number. (c) Cells were transfected with control or SSX2IP siRNA and stained for BBS2 or γ-tubulin and glutamylated (glu) tubulin using indirect immunofluorescence. (d) Quantification of immunofluorescence shown in c. Cells with cilia showing ciliary localization of BBS2 were counted in relation to the overall cell number. (e) Cells were transfected with control or SSX2IP siRNA and stained for BBS2 or γ-tubulin and glutamylated (glu) tubulin using indirect immunofluorescence. (f) Quantification of immunofluorescence shown in c. Cells with cilia showing ciliary localization of BBS2 were counted in relation to the overall cell number. (b, d, f) Mean values from averages from three independent experiments ± SEM (n ≥ 150) normalized to controls. *p < 0.05, **p < 0.01, ***p < 0.001 (two-tailed Student's t test).