FIGURE 6:

SSX2IP knockdown leads to less efficient ciliogenesis and shorter cilia. (a) RPE-1 cells were transfected with control or SSX2IP siRNA and stained for IFT-88, γ-tubulin, or glutamylated (glu) tubulin using indirect immunofluorescence. Magnified merges do not display DAPI staining. (b) Quantification of the relative cilia assembly activity in control and SSX2IP-knockdown cells. Bars show mean values ± SEM from three independent experiments (n ≥ 100 cells). (c) Quantification of cilia lengths in RPE-1 cells after SSX2IP knockdown using the IFT-88, γ-tubulin, and glutamylated tubulin signals. Left (bars), mean values of averages ± SEM from three independent experiments (n ≥ 150) normalized to controls. Right (box-and-whiskers plots), quantification of a single representative experiment. *p < 0.05, **p < 0.01, ***p < 0.001 (two-tailed Student's t test).