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. Author manuscript; available in PMC: 2014 Feb 13.
Published in final edited form as: J Cell Biochem. 2009 Feb 15;106(3):427–443. doi: 10.1002/jcb.22023

Figure 3. Hoxa1-399 inhibits the formation of a DNA-bound Hoxa1-993/Pbx.

Figure 3

(A) EMSA was performed using equal units of in vitro translated Hoxa1-993, Pbx1 and increasing amounts of Hoxa1-399 incubated with 32P-labeled DNA corresponding to the HoxB1-ARE-R3 region of the HoxB1 promoter. Lysate lane (1) indicates endogenous protein present in the rabbit reticulocyte lysate used for the in vitro transcription. (B) Quantitation of EMSA band intensities depicted in (a). Data was normalized to the intensity of the band corresponding to the 993/Pbx complex (lane 2). Error bars are indicative of S.E.M. Data representative of three independent experiments except lane 3 where n=2. ** very significant; indicates a p value of <0.01, when compared to intensity of 993/Pbx1 band.