Figure 6. Response to apoplastic ROS in rcd1 is not influenced by AOX1 or UPOX.

Genotypes studied were Col-0, AOX1a OE (overexpression), AOX1a OE-CA (overexpression of constitutively active AOX1a), the corresponding vector control (VC) and the mutants aox1a, upox1, rcd1-1, rcd1-4, rcd1-1 upox1, rcd1-1 aox1a and rcd1-4 aox1a. Plants were exposed to 6 h of O₃ (350 nL L⁻¹) and after 2 hour recovery in the clean air they were harvested for ion leakage measurements. Samples of untreated plants grown in clean air were simultaneously collected. Clean air and O₃ samples were compared to wild type Col-0 with linear models (P<0.05:*; P<0.01:** and P<0.001:***). Double mutants (rcd1-1 aox1a, rcd1-4 aox1a and rcd1-1 upox1) were also compared to rcd1-1 and rcd1-4. Bars represent means of four to six biological repeats with standard deviation.