Table 1. Infection and dissemination rates of rRVF-wt and rRVF-ΔNSm in Aedes aegypti determined by immunofluorescence assay on sagittal sections of mosquitoes.
rRVF-wt | rRVF-ΔNSm | |||||||||||||||||
Day | Infection Rate | Dissemination Rates | Infection Rate | Dissemination Rates | ||||||||||||||
# pos/# exposed | # pos/# infected | # pos / # exposed | # pos/# infected | |||||||||||||||
(n) | # pos | % pos | (n) | # pos | % pos | (n) | # pos | % pos | (n) | # pos | % pos | (n) | # pos | % pos | (n) | # pos | % pos | |
0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 |
1 | 11 | 7 | 64 | 11 | 0 | 0 | 7 | 0 | 0 | 12 | 0 | 0 | 12 | 0 | 0 | 0 | 0 | 0 |
2 | 11 | 7 | 64 | 11 | 0 | 0 | 7 | 0 | 0 | 10 | 8 | 80 | 10 | 0 | 0 | 8 | 0 | 0 |
3 | 12 | 12 | 100 | 12 | 1 | 8 | 12 | 1 | 8 | 8 | 3 | 38 | 8 | 1 | 13 | 3 | 1 | 33 |
4 | 12 | 10 | 83 | 12 | 0 | 0 | 10 | 0 | 0 | 11 | 3 | 27 | 11 | 1 | 9 | 3 | 1 | 33 |
6 | 12 | 8 | 67 | 12 | 4 | 33 | 8 | 4 | 50 | 8 | 4 | 50 | 8 | 0 | 0 | 4 | 0 | 0 |
8 | 12 | 9 | 75 | 12 | 5 | 42 | 9 | 5 | 56 | 8 | 5 | 63 | 8 | 0 | 0 | 5 | 0 | 0 |
10 | 7 | 7 | 100 | 7 | 7 | 100 | 7 | 7 | 100 | 8 | 6 | 75 | 8 | 0 | 0 | 6 | 0 | 0 |
12 | 7 | 7 | 100 | 7 | 7 | 100 | 7 | 7 | 100 | 4 | 2 | 50 | 4 | 1 | 25 | 2 | 1 | 50 |
14 | 10 | 10 | 100 | 10 | 10 | 100 | 10 | 10 | 100 | 9 | 7 | 78 | 9 | 0 | 0 | 7 | 0 | 0 |
Mosquitoes on day 0 were harvested immediately after receiving an infectious blood meal and were not considered infected at the time of observation since no viral antigen was visible in the midgut epithelial cells.