Table 2. Infection, dissemination, and transmission rates of rRVF-wt and rRVF-ΔNSm in Aedes aegypti 14 days post infection.
| Infection Rate1 * | Dissemination Rates | Transmission Rates | |||||||||||
| # pos/# exposed2 * | # pos/# infected3 * | # pos/# exposed4 * | # pos/# disseminated5 * | ||||||||||
| Virus | Blood meal titer6 | Avg (sd) Day 0 titer7 | Avg (sd) Day 14 titer8 | % pos | (n) | % pos | (n) | % pos | (n) | % pos | (n) | % pos | (n) |
| rRVF-wt | 7.6 | 5.3 (0.04) | 5.5 (0.3) | 84.0 | 25 | 80.0 | 25 | 95.2 | 21 | 60.0 | 25 | 75.0 | 20 |
| rRVF-ΔNSm | 7.9 | 5.4 (0.17) | 2.7 (0.58) | 23.3 | 30 | 0.0 | 30 | 0.0 | 7 | 0.0 | 30 | 0.0 | 0 |
Number of mosquitoes containing detectable virus by plaque assay divided by the number of mosquitoes exposed to infectious blood meal.
De = number of mosquitoes with RVFV antigen in head tissues divided by number of mosquitoes exposed to infectious blood meal.
Di = number of mosquitoes with RVFV antigen in head tissues divided by number of infected mosquitoes.
Te = number of mosquitoes with RVFV-positive saliva by plaque assay divided by number of mosquitoes exposed to infectious blood meal.
Td = number of mosquitoes with RVFV-positive saliva by plaque assay divided by number of mosquitoes with disseminated infection.
Titer expressed as log10 pfu/mL.
n = 3; titer expressed as log10 pfu/mosquito; average is the log10 geometric mean; sd = standard deviation.
rRVFV-wt n = 21; rRVFV-ΔNSm n = 7; titer expressed as log10 pfu/mosquito; average is the log10 geometric mean; sd = standard deviation.
Rate difference between virus types is statistically significant.