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. 2014 Feb 13;8(2):e2703. doi: 10.1371/journal.pntd.0002703

Figure 2. Localization and immunogenicity of SjE16.7.

Figure 2

(A) Expression of SjE16.7 in the egg stage of S. japonicum. Extracts obtained from adult and egg stages of S. japonicum were separated by SDS-PAGE and blotted with anti-SjE16.7 sera. SEA, soluble egg antigen. AwAj, adult worm antigen of S. japonicum. (B) Immunohistological detection of SjE16.7 in S. japonicum eggs. Sections of livers from S. japonicum-infected mice were incubated with normal rabbit sera (a,b) or rabbit anti-SjE16.7 sera (c, d) NRS, normal rabbit sera; α-SjE16.7, rabbit anti-SjE16.7 sera. Right graphs are better seen in the magnification of the insets of upper graphs. Original magnification ×40, scale bar = 50 µm. (C) Precipitate formation around live eggs cultured in vitro with anti-SjE16.7 sera (α-SjE16.7) and S. japonicum infected rabbit sera (S.j.), but not normal rabbit sera (NRS). Original magnification ×40, scale bar = 50 µm. (D) ELISA detection of anit-SEA or SjE16.7 IgG in S. japonicum infected mice. The sera were collected before (Normal) and 42 days after S. japonicum infection (S.j.). SEA, soluble egg antigen; His-SjE16.7, recombinant His-SjE16.7 protein from Pichia yeast; SjE16.7, recombinant SjE16.7 (without GST tag) protein from E. coli. ** P<0.01. (E, F) Time course determined by ELISA. Sera from S. japonicum infected mice (E) or rabbits (F) were collected at different time points. Anti-His-SjE16.7 IgG or IgM antibodies were measured by ELISA. Each group consisted of at least 5 animals. Data are presented as optical density at 450 nm (mean ± SD). ** P<0.01 vs. zero time point.