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. 2014 Feb 13;8(2):e2703. doi: 10.1371/journal.pntd.0002703

Figure 4. SjE16.7 induces neutrophil migration in vitro.

Figure 4

(A) Cell migration was measured using transwell assay. Neutrophils were plated in the upper chamber of the filter that had been coated with fibronectin, and stimulated with denature His-SjE16.7 (95°C, 30 min treatment) or His-SjE16.7 as indicated concentration for 3 h. Cells migrating to the bottom chambers were counted. Soluble egg antigen (SEA, 10 µg/ml) was used as control. Values are means ± S.E.M of 4 independent assays. **P<0.01 vs. group without antigen stimulation. (B) Transwell assays were performed as described in A, except that cells were stimulated with prokaryotic protein GST or GST-SjE16.7 as indicated. Values are means ± S.E.M of 3 independent assays. **P<0.01 vs. group without antigen stimulation. (C) Transwell assays were performed as described in A, except that cells were stimulated with recombinant SjE16.7 with or without purified anti-SjE16.7 antibody (10 µg/ml) as indicated. Values are means ± S.E.M of 2 independent assays. **P<0.01. (D) Transwell assays were performed as described in B, cells were stimulated with recombinant GST or GST-SjE16.7 with or without Polymyxin B (10 µg/ml) as indicated. Values are means ± S.E.M of 2 independent assays. **P<0.01 vs. group without antigen stimulation.