Figure 7. Effect of DAC N-055 on L. tropica parasites.

A: Extracellular L. tropica promastigotes. The survival of extracellular Leishmania promastigotes after 4 d of incubation with DAC N-055 was determined via optical density at 450 nm. The OD values were logarithmically transformed and a curve fit was carried out. The mean of the curve (solid line) and the 95% confidence interval (dashed line) of two independent experiments with 24 OD values per DAC N-055 concentration as well as the ED₅₀ are given. B: Intracellular L. tropica amastigotes. After infection of adherent human monocytes (macrophages, MΦ) for 19 h with stationary phase-grown and opsonized L. tropica promastigotes (MOI = 5) the cells were washed and stimulated for 72 h as indicated. The time point directly after the pulse infection was defined as 0 h. The infection rate and the number of parasites per 100 infected MΦ were determined at time point 0 h and after 72 h of stimulation. The mean ± SEM of three independent experiments are shown. For statistical analysis the Mann-Whitney test was used (** = p<0.001, n. s. = not significant).