Figure 2. Involvement of host's apoptotic pathway in ectopic cell death in Spiroplasma-infected male embryos of D. melanogaster.

(A and B) Quantification of TUNEL-positive area per embryo in control embryos (Ctrl: H99/+ genotype) and apoptosis-deficient embryos (H99: H99/H99 genotype) at stage 11–12 (A) and stage 13–14 (B) (Wilcoxon rank sum test; **, P<0.01). (C–J) TUNEL signals in the Spiroplasma-infected control embryos and apoptosis-deficient embryos at stage 11 (C–F) and stage 13 (G–J). The internal edge of the epidermis is highlighted by a dashed line in (E) and (F).