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. 2014 Feb 13;9(2):e87814. doi: 10.1371/journal.pone.0087814

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© 2014 Köhler et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Pyocyanin, rhamnolipid and elastase production were determined as described in Material and Methods.The defined vqsM deletion mutant of PAO1 (PT1842), and the vqsM mutant (PAO1-UW) of the PAO1 transposon library [39], were not affected in QS-dependent virulence factor expression. When PA2226 was expressed from plasmid pOH13.1, both vqsM mutants showed the same QS-negative phenotype, suggesting that VqsM is not involved in QS-regulation.