Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
A) A target sequence containing LI C-D fusion sites and a LI intron element are combined into a LII 1–2 RNAi vector backbone by BsaI cut-ligation. The chosen BsaI overhangs enable a one-step assembly of a hairpin construct containing the intron element between two copies of the LI C-D target sequence. When expressed, this creates an intron-spliced hairpin RNA, which is effective in gene silencing.
