Skip to main content
. 2014 Feb 13;9(2):e88218. doi: 10.1371/journal.pone.0088218

Figure 5. In planta expression of LIII constructs.

Figure 5

A) Binary plasmids LIII Tri-Color and LIII Tri-Color (neo) were constructed from LII F 1–2 NES-mCherry or LII F1–2 NES-mCherry (neo), LII R 3–4 NLS-CFP, LII F 5–6 Plastid-YFP assemblies and LII insulator fragments LII ins 2–3 and LII ins 4–5 by BpiI cut-ligation into a LIII vector backbone. All LI modules contained in the final LIII construct are depicted under the plasmid map. B) Confocal laser scanning microscopic (CLSM) images of transformed plants. LIII Tri-Color was expressed in N. benthamiana leaves and L. japonicus roots by Agrobacterium mediated transformation. LIII Tri-Color (neo) was used to generate stable transgenic A. thaliana lines. p35S = cauliflower mosaic virus 35S promoter; pUbi = L. japonicus polyubiquitin promoter; NES = nuclear export signal; NLS = nuclear localization signal; 35S-T = cauliflower mosaic virus 35S terminator; HSP-T = heat shock protein terminator of A. thaliana; nos-T = nopaline synthase terminator; neo = neomycin resistance cassette; Plas-M. = Plastid Marker (plastid localized protein of L. japonicus). Scale bars = 25 µm.