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. 2014 Feb 13;9(2):e88479. doi: 10.1371/journal.pone.0088479

Figure 1. 6 and 8mer HA fragments stimulate but HMW HA inhibits rat dermal fibroblast migration in modified scratch wound assays.

Figure 1

Migration of rat dermal fibroblasts was analyzed using Ibidi culture inserts as described in Material and Methods. A: Mixture of HA oligomeres significantly stimulate migration of rat dermal fibroblasts. Cells were treated with DMEM 1% FBS containing a HA oligomere mixture consisting of equal parts of 4mer, 6mer, 8mer and 10mer HA at a final concentration of 10 µg/ml. Cell migration was quantified by measuring the area of cells that migrated into the empty space after removal of the culture insert. Graph represents Mean±SE of N = 6 culture wells B: HMW HA inhibits migration of rat dermal fibroblasts. Cells were treated with DMEM 1% FBS containing 10 µg/ml 500 kDa HA. Cell migration was quantified by measuring the area of cells that migrated into the empty space after removal of the culture insert. Graph represents Mean±SE of N = 6 culture wells C: 6 and 8mer significantly stimulate migration of rat dermal fibroblasts. Cells were treated with DMEM 1% FBS containing either 4mer, 6mer, 8mer, 10mer, 5 kDa, 40 kDa HA at 1 µg/ml or PBS over night. Cell migration was quantified by measuring the area of cells that migrated into the empty space after removal of the culture insert. Graph represents Mean±SE of N = 4 culture wells.