Figure 3. 6mer HA significantly increases TGFβ1 accumulation but has no effect on collagen accumulation during wound repair.

A, B: Cross sections of 7 days old wounds were stained with TGFβ1 specific antibodies as described in Materials and Methods. Positive stained area was quantified by image analysis using ImageJ. Graph shows Mean±SE of N = 18 (6 sections, three areas/section). C: Cross sections of 7 days old wounds were stained with Masson’s Trichrome as described in Materials and Methods. Blue staining (Collagen) was quantified by image analysis using ImageJ. Graph shows Mean±SE of N = 18 (6 sections, three areas/section).