Table 1. The sequences and amplicon sizes of the primer sets used in this study.

Gene	Amplicon size	Primer sequence
MyoD	204 bp	FP: 5′-ggg tac gac acc gcc tac ta-3′
		RP: 5′-gtt ctg tgt cgc tta ggg at-3′
Myogenin	166 bp	FP: 5′-cca gtg aat gca act ccc aca gc-3′
		RP: 5′-aga cat atc ctc cac cgt ga-3′
Myf-5	132 bp	FP: 5′-cct gtc tgg tcc cga aag aac-3′
		RP: 5′-tag acg tga tcc gat cca caa t-3′
Mrf4	190 bp	FP: 5′-gca ccg gct gga tca gca aga g-3′
		RP: 5′-ctg agg cat cca cgt ttg ctc c-3′
Mef2c	175 bp	FP: 5′-gat ggg cg gaga tct gac a-3′
		RP: 5′-gaa cgc gga gat ctg gct tac-3′
Atrogin-1	149 bp	FP: 5′-cag cct gaa cta cga cgt c-3′
		RP: 5′-gct tcc ccc aaa gta cag ta-3′
p21Cip1	135 bp	FP: 5′-gcc gaa aac gga ggc aga c-3′
		RP: 5′-aag atg ggg aag agg cct cct ga-3′
MHC	103 bp	FP: 5′-tgc caa ggg cct gaa tga-3′
		RP: 5′-gct tcc acc taa agg gct gtt-3′
m36b4	73 bp	FP: 5′-ggc agc att tat aac cct gaa gtg-3′
		RP: 5′-cgg aca ccc tcc aga aag c-3′
Gapdh	190 bp	FP: 5′-cct ctg gaa agc tgt ggc gt-3′
		RP: 5′-ttg gca ggt ttc tcc agg cg-3′
FoxO1	97 bp	FP: 5′-tcc cac aca gtg tca aga cta caa-3′
		RP: 5′-ctg ctg tca gac aat ctg aag ga-3′

FP: forward primer.

RP: reverse primer.