Skip to main content
. 2014 Feb 13;9(2):e88704. doi: 10.1371/journal.pone.0088704

Figure 2. Effects of pioglitazone on expression of fetuin-A mRNA in Fao cells.

Figure 2

Fao cells were treated with pioglitazone at different doses (0, 2, 5, and 10 µM for 24 h) (A) or for different periods (0, 6, 12, 18, and 24 h with 10 µM) (B). Total RNA was isolated from Fao cells treated with or without pioglitazone, and the levels of fetuin-A mRNA expression were determined using quantitative real-time RT-PCR. Levels of fetuin-A mRNA were determined using a comparative cycle threshold method, using 18S ribosomal RNA as endogenous reference, and fetuin-A mRNA levels without pioglitazone (A) or at 0 h (B) were defined as 100% (CTL). Mean ± SD were calculated from at least three independent experiments. *P<0.05 vs. CTL.