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. 2014 Feb 13;9(2):e88704. doi: 10.1371/journal.pone.0088704

Figure 6. Effects of pioglitazone on hepatic fetuin-A expression and circulating fetuin-A levels in mice.

Figure 6

Male C57BL/6J mice at 7 weeks of age were divided into a group bred with pioglitazone delivered as a food mixture at 10 mg/kg/day (Pio group: n = 6) and a group without pioglitazone (CTL group: n = 6). Body weight (A) and casual blood glucose levels (B) were monitored weekly in mice in each group from 7 weeks of age until 20 weeks of age. At 20 weeks of age, mice were sacrificed and liver tissues and blood samples were collected. Total RNA was isolated from mice in each group. Fetuin-A mRNA expression was determined using quantitative real-time RT-PCR. Fetuin-A mRNA expression levels were determined using a comparative cycle threshold method, with 18S ribosomal RNA as anendogenous reference. The fetuin-A mRNA level in the CTL group was defined as 100% (C). Circulating fetuin-A levels in the CTL- or Pio group were measured using an ELISA kit (D). Columns represented mean ± SD. *P<0.05 vs. CTL.