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. 2014 Feb 13;9(2):e88629. doi: 10.1371/journal.pone.0088629

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© 2014 Anda et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cells arrested in G1 phase were released in the presence of EdU and immediately UV-irradiated with 1100 J/m². Control cells were not irradiated. Samples were harvested at the time points indicated, fixed, processed, and imaged by fluorescence microscopy. A) Microscopy pictures of EdU -labelled UV-irradiated and control cells 0 and 30 minutes after release. B) Detection of CPD-s by indirect immunflourescence in control and UV-irradiated cells immediately after irradiation. C) The graph shows the percentage of EdU-positive cells in UV-irradiated and control cells at the different time points after release. D) The graph shows the relative fluorescence intensity of CPDs as measured by indirect immunfluorescence in UV-irradiated and control cells at the different time points after release.