Table 1. HRM analysis with MLF-MLR primers of different Leishmania species.

Species	subgenus	Tm peak 1 (°C)	Tm peak 2 (°C)
L. infantum	Leishmania	83.20±0.47	84.32±0.34
L. amazonensis	Leishmania		84.09±0.26
L. guyanensis	Viannia	83.21±0.27
L. panamensis	Viannia	83.07±0.09
L. braziliensis	Viannia	83.08±0.21
L. infantum Plasmid 3	Leishmania		84.02±0.12
L. infantum Plasmid 17	Leishmania	83.34±0.30

Tm values are ± SD.