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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Neurosci. 2013 Dec 1;17(1):89–96. doi: 10.1038/nn.3594

Figure 4.

Figure 4

Behavioral sensitivity and neuroanatomical changes are inherited in F2 and IVF-derived generations. (a,b) Responses of F2-C57Bl/6J males revealed that F2-Ace-C57 mice had an enhanced sensitivity to acetophenone compared with F2-Prop-C57 mice (a). In contrast, F2-Prop-C57 mice had an enhanced sensitivity to propanol compared with F2-Ace-C57 mice (b; F2-Prop-C57, n = 8; F2-Ace-C57, n = 12; OPS to acetophenone: t test, P = 0.0158, t18 = 2.664; OPS to propanol: t test, P = 0.0343, t17 = 2.302). (c–f). F2-Ace-M71 mice whose F0 generation male had been conditioned to acetophenone had larger dorsal and medial M71 glomeruli in the olfactory bulb than F2-Prop-M71 mice whose F0 generation had been conditioned to propanol. Scale bar represents 200 μm. (g) Dorsal M71 glomerular area in F2 generation (M71-LacZ: F2-Prop, n = 7; F2-Ace, n = 8; t test, P < 0.0001, t13 = 5.926). (h) Medial M71 glomerular area in F2 generation (M71-LacZ: F2-Prop, n = 6; F2-Ace, n = 10; t test, P = 0.0006, t14 = 4.44). (i) Dorsal M71 glomerular area in IVF offspring (F1-Prop-IVF, n = 23; F1-Ace-IVF, n = 16; t test, P < 0.001, t37 = 4.083). (j) Medial M71 glomerular area in IVF offspring (F1-Prop-IVF, n = 16; F1-Ace-IVF, n = 19; t test, P < 0.001, t33 = 5.880). Data are presented as mean ± s.e.m.*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.