A, suppression of PTPL1 expression enhances LPA-induced cell migration. pEGFP was co-transfected with pSUPER-siScramble or pSUPER-siPTPL1 into SKOV-3 cells. LPA-induced transwell cell migration was performed as described in Fig. 4B. The immunoblot shows the expression of GFP and endogenous PTPL1 in the whole cell lysates. The result shown is the mean ± S.E. of three independent experiments. *, p < 0.05 versus LPA-stimulated GFP-expressing cells. B, PTPL1 does not significantly affect the ability of TRIP6-Δ-(397–476) to promote LPA-induced cell migration. pEGFP or pEGFP-TRIP6-Δ-(397–476) was co-transafected with pCMV5 or pCMV5-HA-PTPL1 into SKOV-3 cells. LPA-induced transwell cell migration was performed as described above. The results shown are the mean ± S.E. of three independent experiments. *, p < 0.05 versus LPA-stimulated GFP-expressing cells. **, p = 0.08 versus LPA-stimulated GFP-TRIP6-Δ-(397–476)-expressing cells (Student’s t-test).