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. Author manuscript; available in PMC: 2014 Feb 13.
Published in final edited form as: J Biol Chem. 2007 Jun 25;282(33):24381–24387. doi: 10.1074/jbc.M701499200

FIGURE 5. PTPL1 negatively regulates LPA-induced cell migration.

FIGURE 5

A, suppression of PTPL1 expression enhances LPA-induced cell migration. pEGFP was co-transfected with pSUPER-siScramble or pSUPER-siPTPL1 into SKOV-3 cells. LPA-induced transwell cell migration was performed as described in Fig. 4B. The immunoblot shows the expression of GFP and endogenous PTPL1 in the whole cell lysates. The result shown is the mean ± S.E. of three independent experiments. *, p < 0.05 versus LPA-stimulated GFP-expressing cells. B, PTPL1 does not significantly affect the ability of TRIP6-Δ-(397–476) to promote LPA-induced cell migration. pEGFP or pEGFP-TRIP6-Δ-(397–476) was co-transafected with pCMV5 or pCMV5-HA-PTPL1 into SKOV-3 cells. LPA-induced transwell cell migration was performed as described above. The results shown are the mean ± S.E. of three independent experiments. *, p < 0.05 versus LPA-stimulated GFP-expressing cells. **, p = 0.08 versus LPA-stimulated GFP-TRIP6-Δ-(397–476)-expressing cells (Student’s t-test).