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(a) AsPC-1 soft agar growth or (b) HPAF-2 tumorsphere growth was measured in the context of vehicle (DMSO) or 0.1 μM IWP-2 treatment every 72 hours. (c) BAR-luciferase reporter assays at 48 hours in indicated cell lines with stable BAR-luciferase reporter and (d) HPAF tumorsphere growth were measured following treatment every 48 hours with 20 μg/ml anti-WNT7B antibody or matched isotype control antibody. * P<0.05, ** P<0.01.
