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. 2014 Feb 13;9(2):e89009. doi: 10.1371/journal.pone.0089009

Figure 3. IR-induced apoptosis during oogenesis.

Figure 3

The wild type or mutant adult females were irradiated at 40 Gy, and the ovaries were stained by using the TUNEL method to detect the apoptotic cells. (A) The values represent the percentage of wild type or the indicated mutant ovarioles containing TUNEL-positive germline cells in region 2 at 6 h after irradiation. The data are presented as the mean and standard deviation of two independent experiments. The total number of ovarioles counted for each genotype was at least 100. * p = 0.085. ** p = 0.21. (B) The values represent the percentage of wild type ovarioles containing TUNEL-positive germline cells in region 2 (solid line) or degenerating egg chambers at stages 7-10 (dotted line) at 0, 6, 24, 48, 72, and 96 h after irradiation. The data are presented as the mean and standard deviation of two independent experiments. The total number of ovarioles counted at each time point was at least 70. (C) The values represent the percentage of wild type egg chambers at stages 2–6 or 7–10 containing more than 5 TUNEL-positive follicle cells 6 h after irradiation. The total number of egg chambers counted at each time point was at least 150.