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. 2014 Feb 13;9(2):e88934. doi: 10.1371/journal.pone.0088934

Figure 1. Characterization of Vpu-expressing cell lines.

Figure 1

(A) Western blots of lysates of U937-VpuGFP and U937-GFP stable cell lines using anti-Vpu and anti-GFP antibodies. (B) Flow cytometric analysis of GFP expression in U937-VpuGFP and U937-GFP stable cell lines. (C) Flow cytometric analysis of cell surface BST2 and CD4 expression on U937-VpuGFP and U937-GFP stable cell lines. (D) Quantitative RT-PCR of Vpu mRNA levels in U1 cells stably expressing either a Vpu-specific shRNA or a control scrambled shRNA. Error bars represent mean ± SD, n = 3 (*p<0.05). E) Western blot of cell lysates from U1 cells stably expressing either a Vpu-specific shRNA or a control scrambled shRNA, with anti-Vpu antibodies. GAPDH served as a loading control.