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. 2014 Feb 13;9(2):e89025. doi: 10.1371/journal.pone.0089025

Figure 4. Sox9 inhibits BMP2-induced early and late osteogenic differentiation of MSCs in vitro.

Figure 4

(A): C3H10T1/2 cells were infected with AdGFP, AdBMP2 and/or AdSox9. The ALP activities were measured on day 7 and day 9 using ALP histochemical staining (a), and chemiluminescent assays (b). (B): C3H10T1/2 cells were infected with indicated recombinant adenoviruses. On day 11 after infection, the expression of osteopontin (OPN) was assayed by immunocytochemical staining using anti-OPN antibody (a). For matrix mineralization, C3H10T1/2 cells were infected with indicated recombinant adenoviruses and cultured in mineralization medium. Alizarin Red staining were conducted on day 14 after infection (b). Each assay was done in triplicate. ALP assays results are expressed as mean±SD, *P<0.05, # P<0.01.