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. 2014 Feb 13;9(2):e89012. doi: 10.1371/journal.pone.0089012

Figure 3. Effects of platelets on endothelial adhesion molecule expression.

Figure 3

Surface expressions of (A) ICAM-1 (CD54), (B) E-selectin (CD62E) and (C) VCAM-1 (CD106) adhesion molecules on HUVEC following co-culture in direct contact with healthy control (CON; N≥15) PLTs, steady-state sickle cell anemia (SCA; N≥25) PLTs or TNF-α (N≥11). HUVEC (1×106 cells/well) were incubated with CON or SCA PLTs (1×108 PLTs/well) for 4 h (37°C, 5% CO2), or with TNF-α, (10 ng/ml; 3 h, 37°C, 5% CO2). Expression of ICAM-1, E-selectin or VCAM-1 was analyzed on HUVEC (10 000 cells) by flow cytometry using anti-CD54-PE, CD62E-APC and CD106-FITC. **, p<0.01; ***, p<0.001, compared to basal (N≥16).