Skip to main content
. 2014 Feb 13;9(2):e89012. doi: 10.1371/journal.pone.0089012

Figure 4. Transwell inserts reverse the effects of SCA platelets on endothelial adhesion molecules expression.

Figure 4

Surface expressions of (A) ICAM-1 (CD54) and (B) E-selectin (CD62E) adhesion molecules on HUVEC (1×106 cells/well) following culture in the presence of healthy control (CON; N≥6; 1×108 PLTs/well) PLTs or steady-state sickle cell anemia (SCA; N ≥12; 1×108 PLTs/well) PLTs and in the presence and absence of transwell inserts (0.4 µm pores). Expressions of ICAM-1 and E-selectin were evaluated by flow cytometry using anti-CD54-PE and CD62E-APC. *, p<0.01; ***, p<0.001, compared to basal (N = 9).