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. 2014 Feb 13;9(2):e89012. doi: 10.1371/journal.pone.0089012

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© 2014 Proença-Ferreira et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Levels of IL-8 (ng/ml) were quantified in supernatants of co-cultures of HUVEC (1×10⁶ cells/well) (4 h in suspension, 37°C, 5% CO₂) in direct contact with CON (n = 27) or SCA PLTs (1×10⁸ PLTs/well; n = 44), (4 h, 37°C, 5% CO₂); or following pre-incubation of HUVEC with TNF-α (10 ng/ml; N = 24; 3 h, 37°C, 5% CO₂). ***, P<0.001, compared to basal (N = 24); +++, P<0.001, compared to all groups.